PROPOLEOS Y CARIES
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Items 1 - 14 of 14 |
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1: Arch Oral Biol. 2006 Jan;51(1):15-22. Epub 2005 Jul 28. |
The influence of a novel propolis on mutans streptococci biofilms and caries development in rats.
Duarte S, Rosalen PL, Hayacibara MF,
Cury JA, Bowen WH, Marquis RE, Rehder VL, Sartoratto A, Ikegaki M, Koo H.
Eastman Department of Dentistry and Center for
Oral Biology, University of
A flavonoids-free Brazilian propolis (type 6) showed biological effects against mutans streptococci and inhibited the activity of glucosyltransferases. This study evaluated the influence of
the ethanolic extract of a novel type of propolis (EEP) and its purified hexane fraction (EEH) on mutans streptococci biofilms and
the development of dental caries in rats. The chemical composition of the propolis extracts were examined by gas chromatography/mass
spectrometry. The effects of EEP and EEH on Streptococcus mutans
UA159 and Streptococcus sobrinus 6715 biofilms were analysed by time-kill and glycolytic
pH drop assays. Their influence on proton-translocating
F-ATPase activity was also tested. In the animal
study, the rats were infected with S. sobrinus 6715
and fed with cariogenic diet 2000. The rats were
treated topically twice a day with each of the extracts (or control) for 5
weeks. After the experimental period, the microbial composition of their dental
plaque and their caries scores were determined. The results showed that fatty
acids (oleic, palmitic, linoleic
and stearic) were the main compounds identified in
EEP and EEH. These extracts did not show major effects on the viability of mutans streptococci biofilms. However,
EEP and EEH significantly reduced acid production by the biofilms
and also inhibited the activity of F-ATPase (60-65%).
Furthermore, both extracts significantly reduced the incidence of smooth
surface caries in vivo without displaying a reduction of the percentage of S. sobriuns in the animals' plaque (P < 0.05). However,
only EEH was able to reduce the incidence and severity of sulcal
surface caries (P < 0.05). The data suggest that the cariostatic
properties of propolis type 6 are related to its
effect on acid production and acid tolerance of cariogenic
streptococci; the biological activities may be attributed to its high content
of fatty acids.
PMID: 16054589 [PubMed
- in process]
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2:
J Ethnopharmacol. 2005 Oct 3;101(1-3):110-5. |
In vitro and in vivo effects of isolated
fractions of Brazilian propolis on caries
development.
Hayacibara MF, Koo H, Rosalen PL, Duarte S, Franco EM, Bowen WH, Ikegaki M, Cury JA.
Department of Physiological Sciences, Dentistry Faculty of Piracicaba,
State University of Campinas, Av. Limeira,
901 PIRACICABA-SP, CEP: 13414-903, Brazil. mhayacibara@wnet.com.br
Recently, two chemically different types of Brazilian propolis
(type-3 and -12) were shown to have cariostatic
properties. This study aimed to evaluate the influence of their isolated
fractions on mutans streptococci viability, glucosyltransferases (GTFs)
activity and caries development in rats. The ethanolic
extracts of propolis (EEPs)
were serially fractionated into hexane (H-fr), chloroform,
ethyl acetate, and ethanol. The ability of the four fractions and EEP to
inhibit Streptococcus mutans and Streptococcus sobrinus growth and adherence to a glass surface was
examined. The effect on GTFs B and C activity was
also determined. For the caries study, 60 Wistar rats
infected with Streptococcus sobrinus were treated
topically twice daily as follows: (1) EEP type-3, (2) H-fr type-3, (3) EEP type-12, (4) H-fr
type-12, and (5) control. In general, the H-fr from
both types of propolis showed the highest
antibacterial activity and GTFs inhibition. Furthermore,
the EEP and H-fr type-3 and -12 were equally
effective in reducing dental caries in rats. The data suggest that the putative
cariostatic compounds of propolis
type-3 and -12 are mostly non-polar; and H-fr should
be the fraction of choice for identifying further potentially novel anti-caries
agents.
PMID: 15913934 [PubMed
- indexed for MEDLINE]
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3:
Microbiol Res. 2005;160(2):189-95. |
Chemical compositions and antimicrobial
activities of four different Anatolian propolis
samples.
Uzel A, Sorkun K, Oncag O, Cogulu D, Gencay O, Salih B.
Department of Biology, Faculty of Sciences,
Propolis means a gum that is gathered by bees from various plants. It is known
for its biological properties, having antibacterial, antifungal and healing
properties. The aims of this study were to evaluate the antimicrobial activity
of four different Anatolian propolis samples on different
groups of microorganisms including some oral
pathogens and comparison between their chemical compositions. Ethanol extracts
of propolis (EEP) were prepared from four different
Anatolian propolis samples and examined whether EEP
inhibit the growth of the test microorganisms or not.
For the antimicrobial activity assays, minimum inhibitory concentrations (MIC)
were determined by using macrodilution method. The
MIC values of the most effective propolis (TB) were 2
microg/ml for Streptococcus sobrinus
and Enterococcus faecalis,
4 microg/ml for Micrococcus luteus,
Candida albicans and C. krusei,
8 microg/ml for Streptococcus mutans,
Staphylococcus aureus, Staphylococcus epidermidis and Enterobacter aerogenes, 16 microg/ml for
Escherichia coli and C. tropicalis and 32 microg/ml for Salmonella typhimurium
and Pseudomonas aeruginosa. The chemical compositions
of EEP's were determined by high-temperature
high-resolution gas chromatography coupled to mass spectrometry. The main
compounds of four Anatolian propolis samples were flavonoids such as pinocembrin, pinostropin, isalpinin, pinobanksin, quercetin, naringenin, galangine and chrysin. Although propolis
samples were collected from different regions of
PMID: 15881836 [PubMed
- indexed for MEDLINE]
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4:
Biol Pharm Bull.
2004 Nov;27(11):1834-9. |
Comparative evaluation of in-vitro effects of Brazilian green propolis and Baccharis dracunculifolia extracts on cariogenic
factors of Streptococcus mutans.
Leitao
DP, Filho
AA, Polizello
AC, Bastos JK, Spadaro
AC.
Laboratorio de Bioquimica, Faculdade
de Ciencias Farmaceuticas de Ribeirao Preto-Universidade de Sao Paulo, Brazil.
Streptococcus
mutans triggers dental caries establishment by two
major factors: synthesis of organic acids, which demineralize
dental enamel, and synthesis of glucans, which
mediate the attachment of bacteria to the tooth surface. Propolis
is a natural product that may prevent dental caries. Baccharis
dracunculifolia DC (Asteraceae),
a native plant from
PMID: 15516733 [PubMed
- indexed for MEDLINE]
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5:
Hua Xi Kou
Qiang Yi Xue Za Zhi.
2003 Aug;21(4):304-6, 317. |
[Evaluation of the cario-static effect of Nidus vespae on biofilm model in vitro]
[Article in Chinese]
Huang Z, Li J, Zhou X.
Department of Oral Medicine,
OBJECTIVE: To evaluate the Nidus vespae's
cario-static effect on biofilm
model in vitro. METHODS: A four-organism bacterial consortium was grown in a biofilm model on hydroxyapatite
(HA) discs in a continuous culture system and exposed to repeated solution
pulsing respectively. There were three parallel-connected flow cells in the
model, so were the three groups with different solution pulsed in. Negative
control group was pulsed with distilled water, positive
control group was pulsed with 250 mmol/L sucrose
solution as well. While 4.0 g/L Nidus vespae together with 250 mmol/L
sucrose solution was pulsed in the experiment group. During the experiment, the
pH responses against the pulses were recorded. After the 6 pulses, the biofilm surface structure was observed with a scan electron
microscope and the population on the biofilm was
enumerated. RESULTS: Nidus vespae
can significantly inhibit the adherence of Streptococcus mutans
to HA discs compared with the control group of 250 mmol/L
sucrose pulsed in, and can facilitate the remove of acid products. It is also
found that the extra-cellular polysaccharide is reduced with the pulsing of Nidus vespae. CONCLUSION: Nidus vespae in the biofilm model can partially decrease the cariogenic response of sucrose solution pulsed in.
PMID: 14513591 [PubMed
- indexed for MEDLINE]
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6:
Oral
Microbiol Immunol.
2002 Dec;17(6):337-43. |
Effects of apigenin
and tt-farnesol on glucosyltransferase
activity, biofilm viability and caries development in
rats.
Koo H, Pearson SK, Scott-Anne K, Abranches J, Cury JA, Rosalen PL, Park YK, Marquis RE, Bowen WH.
Center for
Oral Biology and Eastman Department of Dentistry, University of
Propolis, a resinous hive product secreted by Apis mellifera bees, has been
shown to reduce the incidence of dental caries in rats. Several compounds,
mainly polyphenolics, have been identified in propolis. Apigenin and tt-farnesol demonstrated biological activity against mutans streptococci. We determined here their effects,
alone or in combination, on glucosyltransferase
activity, biofilm viability, and development of
caries in rats. Sprague-Dawley rats were infected
with Streptococcus sobrinus 6715 and treated
topically twice daily as follows: (1) tt-farnesol,
(2) apigenin, (3) vehicle control, (4) fluoride, (5) apigenin +tt-farnesol, and (6) chlorhexidine. Apigenin (
PMID: 12485324 [PubMed
- indexed for MEDLINE]
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7: J Calif
Dent Assoc. 2001 Dec;29(12):878, 877. |
Caries prevention that's the bees knees.
Horseman
RE.
Publication Types:
PMID: 11813399 [PubMed - indexed for MEDLINE]
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8:
Caries
Res. 1999 Sep-Oct;33(5):393-400. |
Effect of Apis mellifera propolis from two
Brazilian regions on caries development in desalivated
rats.
Koo H, Rosalen PL, Cury JA, Park YK, Ikegaki M, Sattler A.
Department of Physiological Sciences, Faculty of Dentistry of
The purpose of the present study was to evaluate the effect of Apis mellifera propolis collected from two regions of
PMID: 10460964 [PubMed
- indexed for MEDLINE]
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9:
Curr Microbiol. 1998 Jan;36(1):24-8. |
Antimicrobial activity of propolis on oral microorganisms.
Park YK, Koo MH, Abreu JA, Ikegaki M, Cury JA, Rosalen PL.
Formation of dental caries is caused by the colonization and accumulation of
oral microorganisms and extracellular
polysaccharides that are synthesized from sucrose by glucosyltransferase
of Streptococcus mutans. The production of glucosyltransferase from oral microorganisms
was attempted, and it was found that Streptococcus mutans
produced highest activity of the enzyme. Ethanolic
extracts of propolis (EEP) were examined whether EEP
inhibit the enzyme activity and growth of the bacteria or not. All EEP from various
regions in
PMID: 9405742 [PubMed
- indexed for MEDLINE]
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10:
Caries
Res. 1991;25(5):347-51. |
Effects of propolis
on dental caries in rats.
Ikeno K, Ikeno T, Miyazawa C.
Department of Oral Biochemistry,
Propolis, the resinous hive product collected by
bees, is important in the defense of the hive. The
effects of propolis on growth and glucosyltransferase
activity of Streptococcus sobrinus 6715,
Streptococcus mutans PS14 and Streptococcus cricetus OMZ61 in vitro, and on dental caries in rats
infected with S. sobrinus 6715 were investigated. Propolis had antimicrobial activity against S. sobrinus, S. mutans and S. cricetus, and inhibited both water-insoluble glucan synthesis and glucosyltransferase
activity. In rats inoculated with S. sobrinus, about
half of their fissures were carious, while dental caries was significantly less
(p = 0.01) in rats given propolis. Dental caries was
markedly decreased by the multiple actions of propolis
which had antimicrobial activity, inhibited water-insoluble glucan
synthesis, and inhibited glucosyltransferase
activity. No toxic effects of propolis on the growth
of rats were observed under experimental conditions in this study. These
results suggest that propolis can control dental
caries in the rat model system.
PMID: 1836157 [PubMed
- indexed for MEDLINE]
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11:
Stomatol DDR. 1986 Apr;36(4):195-203. |
[Results of a twice-yearly controlled oral hygiene activity using a propolis-containing toothpaste
(double-blind study)]
[Article in German]
Poppe B, Michaelis H.
Publication Types:
PMID: 3462998 [PubMed - indexed for MEDLINE]
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12:
Stomatol Vjesn. 1986;15(3-4):149-52. |
[The effects of propolis on microflora of carious lesion]
[Article in Croatian]
Basic N, Basic F, Konjhodzic H.
PMID: 3506342 [PubMed
- indexed for MEDLINE]
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13:
Stomatologiia (Sofiia).
1969 Sep-Oct;51(5):394-7. |
[Experimenting with Propolis in the treatment
of caries]
[Article in Bulgarian]
Vlaikov M.
PMID: 5267497 [PubMed
- indexed for MEDLINE]
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14: Nauchni Tr Nauchnoizsled Stomatol Inst (Sofiia). 1969;13:47-51. |
[Personal experience with Propolis anesthesia in the treatment of eental
caries]
[Article in Bulgarian]
Mostrova I, Baikusheva V.
PMID: 5274773 [PubMed
- indexed for MEDLINE]